Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice

April 23, 2021 Leave a comment
Within the current research, we examined the significance of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) within the regulation of cardiac operate utilizing genetically modified CaMKIV-null mice. RT-PCR evaluation revealed decreased expression of voltage-dependent calcium channels within the cardiac myocytes of CaMKIV-null mice in contrast with wild-type mice. CaMKIV-null mice confirmed shortened QT time on electrocardiograms.
Pharmacological evaluation revealed decreased responsiveness to the β-adrenergic blocker propranolol in CaMKIV-null mice, whereas the plasma norepinephrine degree was not affected. CaMKIV-null mice confirmed decreased baroreflex on electrocardiograms. Coronary heart price variability evaluation confirmed unstable R-R intervals, a decreased low frequency energy/excessive frequency energy (LF/HF) ratio, and elevated normal deviation of the conventional to regular R-R intervals (SDNN) in CaMKIV-null mice, suggesting decreased responsiveness to β-adrenergic stimulation in CaMKIV-null mice.
Atrial contraction evaluation and cardiac motion potential recording confirmed a decreased response to the β-adrenoceptor agonist isoproterenol in CaMKIV-null mice. Moreover, fluorescence imaging in a CRE-hrGFP assay revealed a decreased response to isoproterenol in CaMKIV-null cardiac myocytes. Taken collectively, our information strongly counsel a big impact of CaMKIV gene ablation on cardiac β-adrenergic sign transduction.

Protein kinase PIM2: A easy PIM household kinase with complicated features in most cancers metabolism and therapeutics

PIM2 (proviral integration website for Moloney murine leukemia virus 2) kinase performs an vital function as an oncogene in a number of cancers, equivalent to leukemia, liver, lung, myeloma, prostate and breast cancers. PIM2 is essentially expressed in each leukemia and strong tumors, and it promotes the transcriptional activation of genes concerned in cell survival, cell proliferation, and cell-cycle development.
Many tumorigenic signaling molecules have been recognized as substrates for PIM2 kinase, and a wide range of inhibitors have been developed for its kinase exercise, together with SMI-4a, SMI-16a, SGI-1776, JP11646 and DHPCC-9. Right here, we summarize the signaling pathways concerned in PIM2 kinase regulation and PIM2 mechanisms in varied neoplastic ailments. We additionally talk about the present standing and future views for the event of PIM2 kinase inhibitors to fight human most cancers, and PIM2 will grow to be a therapeutic goal in cancers sooner or later.

Cyclic AMP-dependent protein kinase reveals antagonistic results on the replication effectivity of various HPV sorts

A number of forms of widespread human papillomaviruses (HPVs) could induce transformation of contaminated cells, scary the event of neoplasms. Two foremost genera of HPVs are categorized as mucosatropic α and cutaneotropic β HPVs, they usually each embody high-risk cancer-associated species. The absence of antiviral medicine has pushed investigations into the main points of the molecular mechanisms of the HPV life cycle. HPV replication is determined by viral helicase E1 and transcription issue E2. Their organic actions are managed by quite a few mobile proteins, together with protein kinases.
Right here, we report that ubiquitously expressed cyclic A
MP-dependent protein kinase A (PKA) differentially regulates the replication of α HPV11, α HPV18 and β HPV5. PKA stimulates the replication of each α HPVs studied however has a extra profound impact on the replication of high-risk α HPV18. Nevertheless, replication of β HPV5 is inhibited by activated PKA in human major keratinocytes and U2OS cells. We present that activation of PKA signaling by totally different pharmacological brokers induces speedy proteasomal degradation of the HPV5 E2 protein, which in flip results in the downregulation of E2-dependent transcription. In distinction, PKA-stimulated induction of HPV18 replication is the results of the downregulation of the E8^E2 transcript coding a potent viral transcriptional inhibitor along with speedy upregulation of E1 and E2 protein ranges.
IMPORTANCEA number of forms of human papillomaviruses (HPVs) are causative brokers of assorted forms of epithelial cancers. Right here, we report that ubiquitously expressed cyclic AMP-dependent protein kinase A (PKA) differentially regulates the replication of assorted forms of HPVs throughout the preliminary amplification and upkeep phases of the viral life cycle. Replication of the pores and skin cancer-related pathogen HPV5 is suppressed, whereas replication of the cervical cancer-associated HPV18 is activated in response to elevated PKA exercise.
Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice
To inhibit HPV5 replication, PKA targets the viral transcriptional activator E2, inducing its speedy proteasomal degradation. PKA-dependent stimulation of HPV18 replication depends on the downregulation of one other E2 gene product, E8^E2, which encodes a potent transcriptional repressor. Our findings spotlight, for the primary time, protein kinase-related mechanistic variations within the regulation of the replication of mucosal and cutaneous HPV sorts.

Interaction Between CMGC Kinases Concentrating on SR Proteins and Viral Replication: Splicing and Past

Protein phosphorylation constitutes a serious post-translational modification that critically regulates the half-life, intra-cellular distribution, and exercise of proteins. Among the many giant variety of kinases that compose the human kinome tree, these focusing on RNA-binding proteins, specifically serine/arginine-rich (SR) proteins, play a serious function within the regulation of gene expression by controlling constitutive and various splicing. In people, these kinases belong to the CMGC [Cyclin-dependent kinases (CDKs), Mitogen-activated protein kinases (MAPKs), Glycogen synthase kinases (GSKs), and Cdc2-like kinases (CLKs)] group and several other research point out that in addition they management viral replication by way of direct or oblique mechanisms.

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1-CSB-E13516h
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  • 1 plate of 96 wells
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Description: Quantitativesandwich ELISA kit for measuring Human Follistatin Like Protein 1(FSTL1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

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Description: Homo sapiens (Human)

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EUR 499
Description: sandwich

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
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EUR 652.8

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
RDR-FSTL1-Hu-96T 96T
EUR 712
Description: sandwich

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
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EUR 907.2

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
SEJ085Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Follistatin Like Protein 1 (FSTL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
SEJ085Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Follistatin Like Protein 1 (FSTL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
SEJ085Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Follistatin Like Protein 1 (FSTL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
SEJ085Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Follistatin Like Protein 1 (FSTL1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
4-SEJ085Hu
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  • 1 plate of 96 wells
  • 5 plates of 96 wells
  • 10 plates of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Follistatin Like Protein 1 (FSTL1) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

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EUR 475
Description: sandwich

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EUR 625.2

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
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EUR 679
Description: sandwich

Human Follistatin Like Protein 1 (FSTL1) ELISA Kit
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EUR 867.6

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Human FSTL1 -Follistatin Like Protein 1- CLIA Kit
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EUR 180
Description: Sandwich

Human FSTL1 -Follistatin Like Protein 1- CLIA Kit
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EUR 546
Description: Sandwich

Human FSTL1 -Follistatin Like Protein 1- CLIA Kit
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EUR 682
Description: Sandwich

Human FSTL1 -Follistatin Like Protein 1- CLIA Kit
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EUR 6820
Description: Sandwich

Human FSTL1 -Follistatin Like Protein 1- CLIA Kit
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EUR 3410
Description: Sandwich

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EUR 2565

Human FSTL1 (Follistatin Like Protein 1) CLIA Kit
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EUR 3124.08

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Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
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EUR 265

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EUR 450

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EUR 535

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EUR 3130

Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
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EUR 350

Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
MBS8802143-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human FSTL1 (Follistatin Like Protein 1) ELISA Kit
MBS8802143-96StripWells 96-Strip-Wells
EUR 445

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SYP-H0092-48wellsplate 48 wells plate
EUR 180

Human FSTL1(Follistatin Like Protein 1) ELISA Kit
SYP-H0092-96wellsplate 96 wells plate
EUR 220

Human FSTL1(Follistatin Like Protein 1) ELISA Kit
RE1085H-48wells 48 wells
EUR 116.55

Human FSTL1(Follistatin Like Protein 1) ELISA Kit
RE1085H-48wellsplate 48 wells plate
EUR 126

Human FSTL1(Follistatin Like Protein 1) ELISA Kit
RE1085H-96wells 96 wells
EUR 161.55

Human FSTL1(Follistatin Like Protein 1) ELISA Kit
RE1085H-96wellsplate 96 wells plate
EUR 180

Human Follistatin Like Protein 1-FSTL1-ELISA Kit
QY-E02219 96T
EUR 400

Human Follistatin Like Protein 1, FSTL1 GENLISA ELISA
KBH2702 1 x 96 wells
EUR 286

Human Follistatin Like Protein 3 ELISA kit
E01A4602 96T
EUR 700
Description: ELISA

Human Follistatin Like Protein 3 ELISA Kit
MBS070723-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Follistatin Like Protein 3 ELISA Kit
MBS070723-48StripWells 48-Strip-Wells
EUR 550

Human Follistatin Like Protein 3 ELISA Kit
MBS070723-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Follistatin Like Protein 3 ELISA Kit
MBS070723-96StripWells 96-Strip-Wells
EUR 765

Human Follistatin Like Protein 3 ELISA kit
E01F0387-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Follistatin Like Protein 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Follistatin Like Protein 3 ELISA kit
E01F0387-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Follistatin Like Protein 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Follistatin Like Protein 3 ELISA kit
E01F0387-48wellsplate 48 wells plate
EUR 280

Human Follistatin Like Protein 3 ELISA kit
E01F0387-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Follistatin Like Protein 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Follistatin Like Protein 3 ELISA kit
E01F0387-96wellsplate 96 wells plate
EUR 405

Human Follistatin Like Protein 3 ELISA Kit
MBS7254159-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human Follistatin Like Protein 3 ELISA Kit
MBS7254159-48StripWells 48-Strip-Wells
EUR 485

Human Follistatin Like Protein 3 ELISA Kit
MBS7254159-5x96StripWells 5x96-Strip-Wells
EUR 3020

Human Follistatin Like Protein 3 ELISA Kit
MBS7254159-96StripWells 96-Strip-Wells
EUR 690

Follistatin Like Protein 1 (FSTL1) Monoclonal Antibody (Human)
4-MAJ085Hu21
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Follistatin Like Protein 1 (FSTL1)

ELISA kit for Human Follistatin Like Protein 1 (FSTL1)
KTE62635-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Human Follistatin Like Protein 1 (FSTL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Follistatin Like Protein 1 (FSTL1)
KTE62635-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Human Follistatin Like Protein 1 (FSTL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Follistatin Like Protein 1 (FSTL1)
KTE62635-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Human Follistatin Like Protein 1 (FSTL1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

CLIA kit for Human FSTL1 (Follistatin Like Protein 1)
E-CL-H0158 1 plate of 96 wells
EUR 700.8
Description: A sandwich CLIA kit for quantitative measurement of Human FSTL1 (Follistatin Like Protein 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human FSTL1 (Follistatin Like Protein 1)
E-EL-H0173 1 plate of 96 wells
EUR 640.8
Description: A sandwich ELISA kit for quantitative measurement of Human FSTL1 (Follistatin Like Protein 1) in samples from Serum, Plasma, Cell supernatant

ELISA kit for Human FSTL1 (Follistatin Like Protein 1)
ELK3142 1 plate of 96 wells
EUR 518.4
Description: A sandwich ELISA kit for detection of Follistatin Like Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Follistatin Like Protein 1 (FSTL1) Monoclonal Antibody (Human), PE
4-MAJ085Hu21-PE
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Follistatin Like Protein 1 (FSTL1). This antibody is labeled with PE.

Follistatin Like Protein 1 (FSTL1) Monoclonal Antibody (Human), APC
4-MAJ085Hu21-APC
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Follistatin Like Protein 1 (FSTL1). This antibody is labeled with APC.

Follistatin Like Protein 1 (FSTL1) Monoclonal Antibody (Human), Cy3
4-MAJ085Hu21-Cy3
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Follistatin Like Protein 1 (FSTL1). This antibody is labeled with Cy3.

Follistatin Like Protein 1 (FSTL1) Monoclonal Antibody (Human), HRP
4-MAJ085Hu21-HRP
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Mouse monoclonal antibody against Human Follistatin Like Protein 1 (FSTL1). This antibody is labeled with HRP.

Follistatin Like Protein 1 (FSTL1) Polyclonal Antibody (Human, Pig)
4-PAJ085Hu01
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  • 20ul
  • 100ul
  • 200ul
  • 1ml
  • 10ml
Description: A Rabbit polyclonal antibody against Human, Pig Follistatin Like Protein 1 (FSTL1)

Follistatin Like Protein 1 (FSTL1) Antibody
abx025769-400l 400 µl
EUR 518.75

Follistatin Like Protein 1 (FSTL1) Antibody
abx025769-400ul 400 ul
EUR 627.6

Follistatin Like Protein 1 (FSTL1) Antibody
abx025769-80l 80 µl
EUR 281.25

Follistatin Like Protein 1 (FSTL1) Antibody
abx102738-100l 100 µl
EUR 287.5

Follistatin Like Protein 1 (FSTL1) Antibody
20-abx102738
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Follistatin Like Protein 1 (FSTL1) Antibody
abx102738-1ml 1 ml
EUR 812.5

Follistatin Like Protein 1 (FSTL1) Antibody
abx102738-200l 200 µl
EUR 362.5

Follistatin Like Protein 1 (FSTL1) Antibody
abx102739-100l 100 µl
EUR 312.5

Follistatin Like Protein 1 (FSTL1) Antibody
20-abx102739
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg

Follistatin Like Protein 1 (FSTL1) Antibody
abx102739-1ml 1 ml
EUR 962.5

Follistatin Like Protein 1 (FSTL1) Antibody
abx102739-200l 200 µl
EUR 400

Follistatin Like Protein 1 (FSTL1) Antibody
abx112569-100l 100 µl
EUR 612.5

Follistatin Like Protein 1 (FSTL1) Antibody
20-abx112569
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  • 50 ul
  • 150 ul

Follistatin Like Protein 1 (FSTL1) Antibody
abx131049-100l 100 µl
EUR 750

Follistatin Like Protein 1 (FSTL1) Antibody
20-abx131049
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  • 10 ug
  • 50 ug
  • 100 ug
  • 200 ug
  • 1 mg
The intention of this evaluate is to explain recognized and rising actions of CMGC kinases that share the frequent property to phosphorylate SR proteins, in addition to their interaction with totally different households of viruses, in an effort to advance towards a complete data of their pro- or anti-viral phenotype and higher assess potential translational alternatives.

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